Bethyl Laboratories
Rabbit anti-RBM28 Antibody Affinity Purified
Primary
Catalog #A303-925A
TargetRBM28
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
RNA-binding protein 28 (RBM28) is a nucleolar component of the spliceosomal ribonucleoprotein complexes that interacts with U1, U2, U4, U5, and U6 spliceosomal small nuclear RNAs (snRNAs). Defects in RBM28 are the cause of alopecia neurologic defects and endocrinopathy syndrome (ANES) [MIM:612079]. Affected individuals have hair loss of variable severity, ranging from complete alopecia to near-normal scalp hair with absence of body hair [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NW13].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 709 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRBM28
Antigen Species
Human
Gene ID55131
Gene Symbol
RBM28
Gene Name
RNA binding motif protein 28
Uniprot IDQ9NW13
Protein Name
RNA-binding protein 28
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RBM28 immobilized on solid support.
The epitope recognized by A303-925A maps to a region between residue 709 and 759 of human RNA Binding Motif Protein 28 using the numbering given in entry NP_060547.2 (GeneID 55131).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-925A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-925A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-925AOpen
Services
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