Bethyl Laboratories
Rabbit anti-RPL5 Antibody Affinity Purified
Primary
Catalog #A303-933A
TargetRPL5
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Ribosomal protein L5 (RPL5) is required for rRNA maturation and formation of the 60S ribosomal subunits. RPL5 binds 5S RNA. Defects in the RPL5 gene are the cause of Diamond-Blackfan anemia type 6 (DBA6), a congenital non-regenerative hypoplastic anemia that usually presents early in infancy [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P46777].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 247 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRPL5
Antigen Species
Human
Gene ID6125
Gene Symbol
RPL5
Gene Name
ribosomal protein L5
Uniprot IDP46777
Protein Name
60S ribosomal protein L5
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RPL5 immobilized on solid support.
The epitope recognized by A303-933A maps to a region between residue 247 and 297 of human Ribosomal Protein L5 using the numbering given in entry NP_000960.2 (GeneID 6125).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 to 1:2000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-933A · Lot 3 (current lot)Open
Product DatasheetPDF · A303-933A · Lot 3 (current lot)Open
Safety Data SheetPDF · A303-933AOpen
Citations / Publications
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