Bethyl Laboratories

Rabbit anti-RPS9 Antibody Affinity Purified

Primary
Catalog #A303-946A
TargetRPS9
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
Detection of human RPS9 by immunohistochemistry.
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Product Description

40S ribosomal protein S9 (RPS9) is a ribosomal protein that is a component of the 40S subunit. RPS9 belongs to the S4P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRPS9
Antigen Species
Human
Gene ID6203
Gene Symbol
RPS9
Gene Name
ribosomal protein S9
Uniprot IDP46781
Protein Name
40S ribosomal protein S9
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to RPS9 immobilized on solid support.

The epitope recognized by A303-946A maps to a region between residue 1 to 50 of human Ribosomal Protein S9 using the numbering given in entry NP_001004.2 (GeneID 6203).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000 - 1:5000

Documents

Product DatasheetPDF · A303-946A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-946A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-946AOpen
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