Bethyl Laboratories
Rabbit anti-MARS Antibody Affinity Purified
Primary
Catalog #A303-960A
TargetMARS
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Methionyl-tRNA synthetase (MARS) is a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [taken from NCBI Entrez Gene (Gene ID: 4141)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMARS
Antigen Species
Human
Gene ID4141
Gene Symbol
MARS
Gene Name
methionyl-tRNA synthetase
Uniprot IDP56192
Protein Name
Methionine--tRNA ligase, cytoplasmic
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MARS immobilized on solid support.
The epitope recognized by A303-960A maps to a region between residue 175 to 225 of human Methionyl-tRNA Synthetase using the numbering given in entry NP_004981.2 (GeneID 4141).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-960A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-960A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-960AOpen
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