Bethyl Laboratories
Rabbit anti-RRP9 Antibody Affinity Purified
Primary
Catalog #A303-970A
TargetRRP9
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Ribosomal RNA processing 9, small subunit (SSU) processome component (RRP9) is a component of a nucleolar small nuclear ribonucleoprotein particle (snoRNP) thought to participate in the processing and modification of pre-ribosomal RNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O43818].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRRP9
Antigen Species
Human
Gene ID9136
Gene Symbol
RRP9
Gene Name
ribosomal RNA processing 9, small subunit (SSU) processome component, homolog (yeast)
Uniprot IDO43818
Protein Name
U3 small nucleolar RNA-interacting protein 2
Alternate Names
ribosomal RNA processing 9, small subunit (SSU) processome component, homolog • RNA, U3 small nucleolar interacting protein 2 • RNU3IP2 • RRP9 homolog • RRP9, small subunit (SSU) processome component, homolog • U3 small nucleolar ribonucleoprotein-associated 55 kDa protein • U3 small nucleolar RNA-interacting protein 2 • U3 snoRNP-associated 55 kDa protein • U3 snoRNP-associated 55-kDa protein • U3-55K
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RRP9 immobilized on solid support.
The epitope recognized by A303-970A maps to a region between residue 175 to 225 of human Ribosomal RNA Processing 9 using the numbering given in entry NP_004695.1 (GeneID 9136).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A303-970A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-970A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-970AOpen
Citations / Publications
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