Bethyl Laboratories
Rabbit anti-Sec62 Antibody Affinity Purified
Primary
Catalog #A303-981A
TargetSec62
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Sec62 and Sec63 protein are found to be associated with ribosome-free Sec61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. Sec62 is an integral membrane protein located in the rough ER [taken from NCBI Entrez Gene (Gene ID: 7095)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 349 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSec62
Antigen Species
Human
Gene ID7095
Gene Symbol
SEC62
Gene Name
SEC62 homolog, preprotein translocation factor
Uniprot IDQ99442
Protein Name
Translocation protein SEC62
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Sec62 immobilized on solid support.
The epitope recognized by A303-981A maps to a region between residue 349 to 399 of human SEC62 using the numbering given in entry NP_003253.1 (GeneID 7095).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-981A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-981A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-981AOpen
Citations / Publications
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