Bethyl Laboratories
Rabbit anti-EDF-1 Antibody Affinity Purified
Primary
Catalog #A304-039A
TargetEDF-1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
Image 1 of 3
local_shipping
Order within 3 hours and 15 minutes for shipment by today
Product Description
Endothelial differentiation-related factor 1 (EDF-1) is a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. EDF-1 has also been found to act as a transcriptional co-activator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators [taken from NCBI Entrez Gene (Gene ID: 8721)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 98 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetEDF-1
Antigen Species
Human
Gene ID8721
Gene Symbol
EDF1
Gene Name
endothelial differentiation related factor 1
Uniprot IDO60869
Protein Name
Endothelial differentiation-related factor 1
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to EDF-1 immobilized on solid support.
The epitope recognized by A304-039A maps to a region between residue 98 to 148 of human Endothelial Differentiation-Related Factor 1 using the numbering given in entry NP_003783.1 (GeneID 8721).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-039A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-039A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-039AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1