Bethyl Laboratories
Rabbit anti-ROR2 Antibody Affinity Purified
Primary
Catalog #A304-242A
TargetROR2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. ROR2 may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development [taken from NCBI Entrez Gene (Gene ID: 4920)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 893 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetROR2
Antigen Species
Human
Gene ID4920
Gene Symbol
ROR2
Gene Name
receptor tyrosine kinase like orphan receptor 2
Uniprot IDQ01974
Protein Name
Tyrosine-protein kinase transmembrane receptor ROR2
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ROR2 immobilized on solid support.
The epitope recognized by A304-242A maps to a region between residue 893 to 943 of human Receptor Tyrosine Kinase-Like Orphan Receptor 2 using the numbering given in entry NP_004551.2 (GeneID 4920).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-242A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-242A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-242AOpen
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