Bethyl Laboratories
Rabbit anti-ALDOA Antibody Affinity Purified
Primary
Catalog #A304-495A
TargetALDOA
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Aldolase A (fructose-bisphosphate aldolase) (ALDOA) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia [taken from NCBI Entrez Gene (Gene ID: 226)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 314 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetALDOA
Antigen Species
Human
Gene ID226
Gene Symbol
ALDOA
Gene Name
aldolase, fructose-bisphosphate A
Uniprot IDP04075
Protein Name
Fructose-bisphosphate aldolase A
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ALDOA immobilized on solid support.
The epitope recognized by A304-495A maps to a region between residue 325 to 375 of human Aldolase A, Fructose-Bisphosphate using the numbering given in entry NP_000025.1 (GeneID 226).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-495A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-495A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-495AOpen
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