Bethyl Laboratories
Rabbit anti-ICLN Antibody Affinity Purified
Primary
Catalog #A304-521A
TargetICLN
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationFlow Cyt, IHC, IP, WB
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Product Description
Chloride channel nucleotide sensitive 1A (ICLN) is a protein that functions in multiple regulatory pathways. ICLN complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization.ICLN is also found associated with the plasma membrane where it functions as a chloride current regulator [taken from NCBI Entrez Gene (Gene ID: 1207)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 187 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetICLN
Antigen Species
Human
Gene ID1207
Gene Symbol
CLNS1A
Gene Name
chloride nucleotide-sensitive channel 1A
Uniprot IDP54105
Protein Name
Methylosome subunit pICln
Product Information
Applications
Flow Cyt, IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ICLN immobilized on solid support.
The epitope recognized by A304-521A maps to a region between residue 187 to 237 of human Chloride Conductance Regulatory Protein ICln using the numbering given in entry NP_001284.1 (GeneID 1207).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.25 µl per 1 x 10^6 cells.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-521A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-521A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-521AOpen
Services
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