Bethyl Laboratories
Rabbit anti-DYNC1I2 Antibody Affinity Purified
Primary
Catalog #A304-529A
TargetDYNC1I2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Cytoplasmic dynein 1 intermediate chain 2 (DYNC1I2) acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. DYNC1I2 is involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q13409].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 588 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDYNC1I2
Antigen Species
Human
Gene ID1781
Gene Symbol
DYNC1I2
Gene Name
dynein cytoplasmic 1 intermediate chain 2
Uniprot IDQ13409
Protein Name
Cytoplasmic dynein 1 intermediate chain 2
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DYNC1I2 immobilized on solid support.
The epitope recognized by A304-529A maps to a region between residue 588 to 638 of human Dynein, Cytoplasmic 1, Intermediate Chain 2 using the numbering given in entry NP_001369.1 (GeneID 1781).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-529A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-529A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-529AOpen
Citations / Publications
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