Bethyl Laboratories
Rabbit anti-DGCR14 Antibody Affinity Purified
Primary
Catalog #A304-566A
TargetDGCR14
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
The gene encoding DGCR14 (DiGeorge syndrome critical region gene 14) is located within the minimal DGS critical region (MDGCR) thought to contain the gene(s) responsible for a group of developmental disorders. These disorders include DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome, and some familial or sporadic conotruncal cardiac defects which have been associated with microdeletion of 22q11.2. The DGCR14 protein may be a component of C complex spliceosomes, and the orthologous protein in the mouse localizes to the nucleus [taken from NCBI Entrez Gene (Gene ID: 8220)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 426 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDGCR14
Antigen Species
Human
Gene ID8220
Gene Symbol
ESS2
Gene Name
DiGeorge syndrome critical region gene 14
Uniprot IDQ96DF8
Protein Name
Protein DGCR14
Alternate Names
bis1 • DGCR13 • DGCR14 • DGSH • DGS-H • DGSI • DGS-I • DiGeorge syndrome critical region 13 • DiGeorge syndrome critical region 14 • DiGeorge syndrome critical region gene 13 • DiGeorge syndrome critical region gene 14 • DiGeorge syndrome critical region gene DGSI • DiGeorge syndrome gene H • DiGeorge syndrome gene I • diGeorge syndrome protein H • ES2 • Es2el • ESS-2 • Protein DGCR13 • protein DGCR14 • Protein ES2 • splicing factor ESS-2 homolog
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DGCR14 immobilized on solid support.
The epitope recognized by A304-566A maps to a region between residue 426 to 476 of human DiGeorge Syndrome Critical Region Gene 14 using the numbering given in entry NP_073210.1 (GeneID 8220).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:500 - 1:2500
Documents
Product DatasheetPDF · A304-566A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-566A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-566AOpen
Citations / Publications
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