Bethyl Laboratories
Rabbit anti-COPB1 Antibody Affinity Purified
Primary
Catalog #A304-724A
TargetCOPB1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
COPB1 is a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum [taken from NCBI Entrez Gene (Gene ID: 1315)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 903 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCOPB1
Antigen Species
Human
Gene ID1315
Gene Symbol
COPB1
Gene Name
coatomer protein complex subunit beta 1
Uniprot IDP53618
Protein Name
Coatomer subunit beta
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to COPB1 immobilized on solid support.
The epitope recognized by A304-724A maps to a region between residue 903 to 853 of human Coatomer Protein Complex, Subunit Beta 1 using the numbering given in entry NP_001137533.1 (GeneID 1315).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-724A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-724A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-724AOpen
Citations / Publications
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