Bethyl Laboratories
Rabbit anti-CHC Antibody Affinity Purified
Primary
Catalog #A304-743A
TargetCHC
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains [taken from NCBI Entrez Gene (Gene ID: 1213)].www.uniprot.org/uniprot/Q00610].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1625 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCHC
Antigen Species
Human
Gene ID1213
Gene Symbol
CLTC
Gene Name
clathrin heavy chain
Uniprot IDQ00610
Protein Name
Clathrin heavy chain 1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CHC immobilized on solid support.
The epitope recognized by A304-743A maps to a region between residue 1625 to 1675 of human Clathrin, Heavy Chain using the numbering given in entry NP_004850.1 (GeneID 1213).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-743A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-743A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-743AOpen
Citations / Publications
Services
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