Bethyl Laboratories
Rabbit anti-EIF3L Antibody Affinity Purified
Primary
Catalog #A304-754A
TargetEIF3L
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
Image 1 of 2
local_shipping
Order within 6 hours and 23 minutes for shipment by today
Product Description
Eukaryotic translation initiation factor 3 subunit L (EIF3L) is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y262].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 514 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetEIF3L
Antigen Species
Human
Gene ID51386
Gene Symbol
EIF3L
Gene Name
eukaryotic translation initiation factor 3 subunit L
Uniprot IDQ9Y262
Protein Name
Eukaryotic translation initiation factor 3 subunit L
Alternate Names
eIEF associated protein HSPC021 • EIF3EIP • eIF3l • EIF3S11 • EIF3S6IP • eukaryotic translation initiation factor 3 subunit 6-interacting protein • eukaryotic translation initiation factor 3 subunit E-interacting protein • eukaryotic translation initiation factor 3 subunit L • HSPC021 • HSPC025 • MSTP005
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to EIF3L immobilized on solid support.
The epitope recognized by A304-754A maps to a region between residue 514 to 564 of human Eukaryotic Translation Initiation Factor 3 Subunit L using the numbering given in entry Q9Y262.1 (GeneID 51386).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-754A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-754A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-754AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1