Bethyl Laboratories
Rabbit anti-Catalase/CAT Antibody Affinity Purified
Primary
Catalog #A304-767A
TargetCatalase/CAT
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
CAT is a catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers [taken from NCBI Entrez Gene (Gene ID: 847)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 477 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCatalase/CAT
Antigen Species
Human
Gene ID847
Gene Symbol
CAT
Gene Name
catalase
Uniprot IDP04040
Protein Name
Catalase
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Catalase/CAT immobilized on solid support.
The epitope recognized by A304-767A maps to a region between residue 477 to 527 of human Catalase using the numbering given in entry P04040 (GeneID 847).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A304-767A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-767A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-767AOpen
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