Bethyl Laboratories
Rabbit anti-Aspartyl-tRNA Synthetase/DARS Antibody Affinity Purified
Primary
Catalog #A304-799A
TargetAspartyl-tRNA Synthetase/DARS
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
Image 1 of 2
local_shipping
Order within 21 hours and 23 minutes for shipment by Tuesday, November 3
Product Description
Aspartyl-tRNA Synthetase/DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14868].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
Antigen Species
Human
Gene ID1615
Gene Symbol
DARS
Gene Name
aspartyl-tRNA synthetase
Uniprot IDP14868
Protein Name
Aspartate--tRNA ligase, cytoplasmic
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Aspartyl-tRNA Synthetase/DARS immobilized on solid support.
The epitope recognized by A304-799A maps to a region between residue 25 to 75 of human Aspartyl-tRNA synthetase using the numbering given in entry P14868.2 (GeneID 1615).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-799A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-799A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-799AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1