Bethyl Laboratories
Rabbit anti-ALDH18A1/P5CS Antibody Affinity Purified
Primary
Catalog #A304-802A
TargetALDH18A1/P5CS
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
ALDH18A1/P5CS is a bifunctional enzyme that converts glutamate to glutamate 5-semialdehyde, an intermediate in the biosynthesis of proline, ornithine and arginine [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P54886].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 575 and 625
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetALDH18A1/P5CS
Antigen Species
Human
Gene ID5832
Gene Symbol
ALDH18A1
Gene Name
aldehyde dehydrogenase 18 family member A1
Uniprot IDP54886
Protein Name
Delta-1-pyrroline-5-carboxylate synthase
Alternate Names
ADCL3 • aldehyde dehydrogenase family 18 member A1 • ARCL3A • delta1-pyrroline-5-carboxlate synthetase • delta-1-pyrroline-5-carboxylate synthase • delta-1-pyrroline-5-carboxylate synthetase • Gamma-glutamyl kinase • Gamma-glutamyl phosphate reductase (GPR) • Glutamate 5-kinase (GK) • Glutamate-5-semialdehyde dehydrogenase • Glutamyl-gamma-semialdehyde dehydrogenase • GSAS • P5CS • PYCS • pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase) • spastic paraplegia 9 (autosomal dominant) • Spastic paraplegia-9 (spastic paraparesis with amyotrophy, cataracts and gastroesophageal reflux) • SPG9 • SPG9A • SPG9B
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ALDH18A1/P5CS immobilized on solid support.
The epitope recognized by A304-802A maps to a region between residue 575 to 625 of human Aldehyde Dehydrogenase 18 Family, Member A1 using the numbering given in entry P54886.2 (GeneID 5832).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A304-802A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-802A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-802AOpen
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