Bethyl Laboratories
Rabbit anti-SF3A2/SAP62 Antibody Affinity Purified
Primary
Catalog #A304-821A
TargetSF3A2/SAP62
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
SF3A2 is subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein [taken from NCBI Entrez Gene (Gene ID: 8175)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSF3A2/SAP62
Antigen Species
Human
Gene ID8175
Gene Symbol
SF3A2
Gene Name
splicing factor 3a subunit 2
Uniprot IDQ15428
Protein Name
Splicing factor 3A subunit 2
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SF3A2/SAP62 immobilized on solid support.
The epitope recognized by A304-821A maps to a region between residue 1 to 50 of human Splicing factor 3A subunit 2 using the numbering given in entry Q15428.2 (GeneID 8175).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A304-821A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-821A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-821AOpen
Citations / Publications
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