Bethyl Laboratories

Rabbit anti-CD2BP2 Antibody Affinity Purified

Primary
Catalog #A304-826A
TargetCD2BP2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Detection of human CD2BP2 by western blot of immunoprecipitates.
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Product Description

CD2BP2 is involved in pre-mRNA splicing as component of the U5 snRNP complex that is involved in spliceosome assembly [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O95400].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCD2BP2
Antigen Species
Human
Gene ID10421
Gene Symbol
CD2BP2
Gene Name
CD2 cytoplasmic tail binding protein 2
Uniprot IDO95400
Protein Name
CD2 antigen cytoplasmic tail-binding protein 2
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to CD2BP2 immobilized on solid support.

The epitope recognized by A304-826A maps to a region between residue 250 to 300 of human CD2 antigen cytoplasmic tail-binding protein 2 using the numbering given in entry O95400.1 (GeneID 10421).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A304-826A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-826A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-826AOpen
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