Bethyl Laboratories
Rabbit anti-WBP11/NPWBP Antibody Affinity Purified
Primary
Catalog #A304-855A
TargetWBP11/NPWBP
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
WW domain-binding protein 11 (WBP11) activates pre-mRNA splicing. It may inhibit PP1 phosphatase activity [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y2W2].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetWBP11/NPWBP
Antigen Species
Human
Gene ID51729
Gene Symbol
WBP11
Gene Name
WW domain binding protein 11
Uniprot IDQ9Y2W2
Protein Name
WW domain-binding protein 11
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to WBP11/NPWBP immobilized on solid support.
The epitope recognized by A304-855A maps to a region between residue 175 to 225 of human WW domain-binding protein 11 using the numbering given in entry Q9Y2W2.1 (GeneID 51729).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A304-855A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-855A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-855AOpen
Citations / Publications
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