Bethyl Laboratories
Rabbit anti-AIMP1/EMAPII/SCYE1 Antibody Affinity Purified
Primary
Catalog #A304-896A
TargetAIMP1/EMAPII/SCYE1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (AIMP1) is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cell [taken from NCBI Entrez Gene (Gene ID: 9255)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 262 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAIMP1/EMAPII/SCYE1
Antigen Species
Human
Gene ID9255
Gene Symbol
AIMP1
Gene Name
aminoacyl tRNA synthetase complex interacting multifunctional protein 1
Uniprot IDQ12904
Protein Name
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
Alternate Names
aminoacyl tRNA synthase complex-interacting multifunctional protein 1 • ARS-interacting multifunctional protein 1 • EMAP2 • EMAPII • endothelial monocyte-activating polypeptide 2 • endothelial-monocyte activating polypeptide II • HLD3 • multisynthase complex auxiliary component p43 • multisynthetase complex auxiliary component p43 • p43 • SCYE1 • small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AIMP1/EMAPII/SCYE1 immobilized on solid support.
The epitope recognized by A304-896A maps to a region between residue 262 to 312 of human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (Multisynthase complex auxiliary component p43) [Cleaved into: Endothelial monocyte-activating polypeptide 2 (EMAP-2) (Endothelial monocyte-activating polypeptide II) (EMAP-II) (Small inducible cytokine subfamily E member 1)] using the numbering given in entry Q12904 (GeneID 9255).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-896A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-896A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-896AOpen
Citations / Publications
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