Bethyl Laboratories
Rabbit anti-CNOT10 Antibody Affinity Purified
Primary
Catalog #A304-899A
TargetCNOT10
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
CCR4-NOT transcription complex subunit 10 (CNOT10) is a component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H9A5].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 600 and 650
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCNOT10
Antigen Species
Human
Gene ID25904
Gene Symbol
CNOT10
Gene Name
CCR4-NOT transcription complex subunit 10
Uniprot IDQ9H9A5
Protein Name
CCR4-NOT transcription complex subunit 10
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CNOT10 immobilized on solid support.
The epitope recognized by A304-899A maps to a region between residue 600 to 650 of human CCR4-NOT transcription complex subunit 10 using the numbering given in entry Q9H9A5.1 (GeneID 25904).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A304-899A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-899A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-899AOpen
Citations / Publications
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