Bethyl Laboratories
Rabbit anti-CWC15 Antibody Affinity Purified
Primary
Catalog #A304-901A
TargetCWC15
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
CWC15 is a component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9P013].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCWC15
Antigen Species
Human
Gene ID51503
Gene Symbol
CWC15
Gene Name
CWC15 spliceosome associated protein homolog
Uniprot IDQ9P013
Protein Name
Spliceosome-associated protein CWC15 homolog
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CWC15 immobilized on solid support.
The epitope recognized by A304-901A maps to a region between residue 75 to 125 of human Protein CWC15 homolog using the numbering given in entry Q9P013.2 (GeneID 51503).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A304-901A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-901A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-901AOpen
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