Bethyl Laboratories
Rabbit anti-ATP5A1 Antibody Affinity Purified
Primary
Catalog #A304-939A
TargetATP5A1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
ATP synthase subunit alpha mitochondrial (ATP5A1) is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c) [taken from NCBI Entrez Gene (Gene ID: 498)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetATP5A1
Antigen Species
Human
Gene ID498
Gene Symbol
ATP5F1A
Gene Name
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Uniprot IDP25705
Protein Name
ATP synthase subunit alpha, mitochondrial
Alternate Names
ATP synthase alpha chain, mitochondrial • ATP synthase F1 subunit alpha • ATP synthase subunit alpha, mitochondrial • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 • ATP sythase (F1-ATPase) alpha subunit • ATP5A • ATP5A1 • ATP5AL2 • ATPM • COXPD22 • epididymis secretory sperm binding protein Li 123m • hATP1 • HEL-S-123m • MC5DN4 • mitochondrial ATP synthetase, oligomycin-resistant • MOM2 • OMR • ORM
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ATP5A1 immobilized on solid support.
The epitope recognized by A304-939A maps to a region between residue 100 to 150 of human ATP synthase subunit alpha, mitochondrial using the numbering given in entry P25705.1 (GeneID 498).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:2000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A304-939A · Lot 2 (current lot)Open
Product DatasheetPDF · A304-939A · Lot 2 (current lot)Open
Safety Data SheetPDF · A304-939AOpen
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