Bethyl Laboratories

Rabbit anti-RPS15A Antibody Affinity Purified

Primary
Catalog #A304-990A
TargetRPS15A
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
Detection of human and mouse RPS15A by western blot.
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Product Description

Belongs to the ribosomal protein S8P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS15A is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8P family of ribosomal proteins. It is located in the cytoplasm [taken from NCBI Entrez Gene (Gene ID: 6210)].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRPS15A
Antigen Species
Human
Gene ID6210
Gene Symbol
RPS15A
Gene Name
ribosomal protein S15a
Uniprot IDP62244
Protein Name
40S ribosomal protein S15a
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to RPS15A immobilized on solid support.

The epitope recognized by A304-990A maps to a region between residue 1 to 50 of human 40S ribosomal protein S15a using the numbering given in entry P62244.2 (GeneID 6210).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:2000 - 1:10,000

Documents

Product DatasheetPDF · A304-990A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-990A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-990AOpen

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