Bethyl Laboratories
Rabbit anti-RPL29/ Ribosomal Protein L29 Antibody Affinity Purified
Primary
Catalog #A305-056A
TargetRPL29/Ribosomal Protein L29
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 2
local_shipping
Order within 5 hours and 48 minutes for shipment by today
Product Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL29 is a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29E family of ribosomal proteins. The protein is also a peripheral membrane protein expressed on the cell surface that directly binds heparin [taken from NCBI Entrez Gene (Gene ID: 6159)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 109 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
Antigen Species
Human
Gene ID6159
Gene Symbol
RPL29
Gene Name
ribosomal protein L29
Uniprot IDP47914
Protein Name
60S ribosomal protein L29
Alternate Names
60S ribosomal protein L29 • cell surface heparin-binding protein HIP • heparin/heparan sulfate-binding protein • heparin/heparan sulfate-interacting protein • HIP • HP/HS-interacting protein • HUMRPL29 • L29 • large ribosomal subunit protein eL29 • ribosomal protein YL43 homologue • RPL29_3_370 • RPL29P10
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RPL29/ Ribosomal Protein L29 immobilized on solid support.
The epitope recognized by A305-056A maps to a region between residue 109 to 159 of human 60S ribosomal protein L29 using the numbering given in entry P47914.2 (GeneID 6159).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A305-056A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-056A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-056AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1