Bethyl Laboratories
Rabbit anti-RPL8/Ribosomal Protein L8 Antibody Affinity Purified
Primary
Catalog #A305-059A
TargetRPL8/Ribosomal Protein L8
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
The gene RPL8 encodes 60S ribosomal protein L8 that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins and is located in the cytoplasm. In rat, the protein associates with the 5. 8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface [taken from NCBI Entrez Gene (Gene ID: 6132)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
Antigen Species
Human
Gene ID6132
Gene Symbol
RPL8
Gene Name
ribosomal protein L8
Uniprot IDP62917
Protein Name
60S ribosomal protein L8
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RPL8/Ribosomal Protein L8 immobilized on solid support.
The epitope recognized by A305-059A maps to a region between residue 100 to 150 of human 60S ribosomal protein L8 using the numbering given in entry P62917.2 (GeneID 6132).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-059A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-059A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-059AOpen
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