Bethyl Laboratories
Rabbit anti-YARS/Tyrosyl-tRNA Synthetase/TYRRS Antibody Affinity Purified
Primary
Catalog #A305-064A
TargetYARS/Tyrosyl-tRNA Synthetase/TYRRS
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
The YARS gene encodes the protein Tyrosine--tRNA ligase, cytoplasmic, also known as Tyrosyl-tRNA synthetase. Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution [taken from NCBI Entrez Gene (Gene ID: 8565)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
Antigen Species
Human
Gene ID8565
Gene Symbol
YARS
Gene Name
tyrosyl-tRNA synthetase
Uniprot IDP54577
Protein Name
Tyrosine--tRNA ligase, cytoplasmic
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to YARS/Tyrosyl-tRNA Synthetase/TYRRS immobilized on solid support.
The epitope recognized by A305-064A maps to a region between residue 1 to 50 of human Tyrosyl-tRNA synthetase, cytoplasmic using the numbering given in entry P54577.4 (GeneID 8565).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-064A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-064A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-064AOpen
Citations / Publications
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