Bethyl Laboratories
Rabbit anti-RNPC3 Antibody Affinity Purified
Primary
Catalog #A305-145A
TargetRNPC3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
RNA-binding protein 40, encoded for by RNPC3, participates in pre-mRNA U12-dependent splicing, performed by the minor spliceosome which removes U12-type introns [taken from UniProtKB (Entry: Q96LT9)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRNPC3
Antigen Species
Human
Gene ID55599
Gene Symbol
RNPC3
Gene Name
RNA binding region (RNP1, RRM) containing 3
Uniprot IDQ96LT9
Protein Name
RNA-binding protein 40
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RNPC3 immobilized on solid support.
The epitope recognized by A305-145A maps to a region between residue 250 to 300 of human RNA-binding protein 40 using the numbering given in entry Q96LT9.1 (GeneID 55599).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A305-145A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-145A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-145AOpen
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