Bethyl Laboratories

Rabbit anti-ERP29 Antibody Affinity Purified

Primary
Catalog #A305-406A
TargetERP29
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
Detection of human ERP29 by western blot.
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Product Description

Endoplasmic reticulum resident protein 29 (ERP29) is a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER [taken from NCBI Entrez Gene (Gene ID: 10961)].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetERP29
Antigen Species
Human
Gene ID10961
Gene Symbol
ERP29
Gene Name
endoplasmic reticulum protein 29
Uniprot IDP30040
Protein Name
Endoplasmic reticulum resident protein 29
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to ERP29 immobilized on solid support.

The epitope recognized by A305-406A maps to a region between residue 75 to 125 of human Endoplasmic reticulum resident protein 29 using the numbering given in entry P30040.4 (GeneID 10961).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:2000 - 1:10,000

Documents

Product DatasheetPDF · A305-406A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-406A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-406AOpen
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