Bethyl Laboratories
Rabbit anti-DCPS Antibody Affinity Purified
Primary
Catalog #A305-444A
TargetDCPS
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
DCPS is a decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. DCPS Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP)[taken from NCBI Entrez Gene (Gene ID: 28960)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 287 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDCPS
Antigen Species
Human
Gene ID28960
Gene Symbol
DCPS
Gene Name
decapping enzyme, scavenger
Uniprot IDQ96C86
Protein Name
m7GpppX diphosphatase
Alternate Names
5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase • ARS • DCS1 • DCS-1 • decapping scavenger enzyme • epididymis secretory sperm binding protein • heat shock-like protein 1 • HINT5 • HINT-5 • hint-related 7meGMP-directed hydrolase • histidine triad nucleotide-binding protein 5 • histidine triad protein member 5 • homolog of C. elegans 7meGMP-directed hydrolase dcs-1 • HSL1 • HSPC015 • m7GpppX diphosphatase • mRNA decapping enzyme • scavenger mRNA-decapping enzyme DcpS
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DCPS immobilized on solid support.
The epitope recognized by A305-444A maps to a region between residue 287 to 337 of human Scavenger mRNA-decapping enzyme DcpS using the numbering given in entry Q96C86.2 (GeneID 28960).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-444A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-444A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-444AOpen
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