Bethyl Laboratories
Rabbit anti-CHCHD3 Antibody Affinity Purified
Primary
Catalog #A305-497A
TargetCHCHD3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
Image 1 of 2
local_shipping
Order within 11 hours and 41 minutes for shipment by today
Product Description
CCoiled-coil-helix-coiled-coil-helix domain-containing protein 3 (CHCHD3) is an inner mitochondrial membrane scaffold protein. Absence of CHCHD3 affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. CHCHD3 is part of the mitochondrial contact site and cristae organizing system (MICOS) [taken from NCBI Entrez Gene (Gene ID: 54927)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 177 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCHCHD3
Antigen Species
Human
Gene ID54927
Gene Symbol
CHCHD3
Gene Name
coiled-coil-helix-coiled-coil-helix domain containing 3
Uniprot IDQ9NX63
Protein Name
MICOS complex subunit MIC19
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CHCHD3 immobilized on solid support.
The epitope recognized by A305-497A maps to a region between residue 177 to 227 of human Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial using the numbering given in entry Q9NX63.1 (GeneID 54927).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A305-497A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-497A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-497AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1