Bethyl Laboratories

Rabbit anti-CNDP2 Antibody Affinity Purified

Primary
Catalog #A305-565A
TargetCNDP2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
Detection of human CNDP2 by western blot of immunoprecipitates.
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Product Description

CNDP2, also known as tissue carnosinase and peptidase A (EC 3.4.13.18), is a nonspecific dipeptidase rather than a selective carnosinase (Teufel et al., 2003 [PubMed 12473676]) [taken from NCBI Entrez Gene (Gene ID: 55748)].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCNDP2
Antigen Species
Human
Gene ID55748
Gene Symbol
CNDP2
Gene Name
CNDP dipeptidase 2 (metallopeptidase M20 family)
Uniprot IDQ96KP4
Protein Name
Cytosolic non-specific dipeptidase
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to CNDP2 immobilized on solid support.

The epitope recognized by A305-565A maps to a region between residue 100 to 150 of human Cytosolic non-specific dipeptidase using the numbering given in entry Q96KP4.2 (GeneID 55748).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000

Documents

Product DatasheetPDF · A305-565A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-565A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-565AOpen
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