Goat anti-ADAMTS13 Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to ADAMTS13 immobilized on solid support.

The epitope recognized by A301-003A maps to a region between residue 1377 and the C-terminus (residue 1427) of human ADAM (a disintegrin-like and metalloprotease) metallopeptidase with thrombospondin type 1 motif, 13 using the numbering given in entry NP_620594.1 (GeneID 11093).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).