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Goat anti-Biotin Antibody HRP Conjugated
Bethyl Laboratories
Catalog #
Target:
Biotin
Applications:
ELISA,
ICC,
IHC,
WB
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
HRP,
Unconjugated
Purity:
Antigen Affinity Purified
Product Details
Specifications
Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt,
2 years from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change
Production Procedures
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with KLH-biotin and BSA-biotin. There is no reaction with free KLH or BSA. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with KLH-biotin and BSA-biotin. There is no reaction with free KLH or BSA.
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with KLH-biotin and BSA-biotin. There is no reaction with free KLH or BSA. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with KLH-biotin and BSA-biotin. There is no reaction with free KLH or BSA.
Country of Origin
USA
Additional Product Information
Biotin, also known as Vitamin B7, is primarily known for its role in mammalian metabolism as a coenzyme. In molecular biology, biotin can be conjugated to molecules to serve as a binding partner for streptavidin.
Applications
Not all listed applications have been specifically tested by our laboratory.