Bethyl Laboratories
Goat anti-DNP Antibody Affinity Purified
Primary
Catalog #A150-117A
TargetDNP
HostGoat
ClonalityPolyclonal
ReactivitySpecies Independent
ApplicationELISA, ICC, IHC, WB

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Product Description
2,4-Dinitrophenol (DNP) is an ionophore that affects the protein gradient across the mitochondrial membrane, making ATP energy production less efficient. Historically, the molecule is used to help increase weight loss by increasing the overall metabolic rate. However, it has shown unacceptably high side effects and is not considered safe for human consumption.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Species Independent
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Target Identity
TargetDNP
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
This antibody reacts with DNP conjugated proteins in precipitation and ELISA techniques. No reaction with KLH was detectable.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A150-117AOpen
Product DatasheetPDF · A150-117A · Lot 250401 (current lot)Open
Citations / Publications
Services
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Antibody and conjugate formats developed together, built to your specification.
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Full custom monoclonal development, from immunization through validation.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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