Bethyl Laboratories
Goat anti-FITC Antibody Affinity Purified
Primary
Catalog #A150-112A
TargetFITC
HostGoat
ClonalityPolyclonal
ReactivitySpecies Independent
ApplicationELISA, ICC, IHC, WB

Product Description
Fluorescein isothiocyanate (FITC) is a fluorescent conjugate that is used to label amine-containing molecules like proteins and peptides. This molecule has an excitation wavelength of 491 nm and an emission wavelength of 516 nm.
Specifications
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Reactivity
Species Independent
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1 mg/ml
Target Identity
TargetFITC
Product Information
Applications
ELISA, ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
2 years from date of receipt
Country of Origin
USA
Production & Epitope
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis this antibody reacts specifically with FITC. There is no reaction with KLH.
Applications
Not all listed applications have been specifically tested by our laboratory.
Enzyme-Linked Immunosorbent Assay (ELISA)1:1000 - 1:30,000; for coating plates 1:100 - 1:500
Immunocytochemistry (ICC)1:200 - 1:2000
Immunohistochemistry (IHC)1:200 - 1:2000
Western Blot (WB)1:1000 - 1:30,000
Documents
Safety Data SheetPDF · A150-112AOpen
Product DatasheetPDF · A150-112A · Lot 260108 (current lot)Open
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
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Full custom monoclonal development, from immunization through validation.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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