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Goat anti-HA Tag Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Target:
HA Tag
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
HRP, Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.1 mg)

HRP (0.1 mg)

Agarose Immobilized (0.1 mg)

$268.00 $348.00
Qty:

Product Details

Specifications

Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change
Production Procedures
Goats were immunized with HA (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Goats were immunized with HA (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support and conjugated to horseradish peroxidase (HRP).

Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
Goats were immunized with HA (YPYDVPDYA) conjugated to KLH.

Antibody was affinity purified using the peptide immobilized on solid support. Goat anti-HA affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method. Final concentration is 0.1 mg antibody in 0.4 ml of 50% slurry containing 0.2 ml agarose.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4, before coupling to agarose beads.
Country of Origin
USA

Additional Product Information

The HA tag is an epitope tag with a 9-residue sequence of YPYDVPDYA. Derived from human influenza hemagglutinin surface glycoprotein, HA tags can help with detection of proteins in western blot, IP, or microscopic techniques.

Applications

Not all listed applications have been specifically tested by our laboratory. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).