Goat anti-Human Albumin Antibody Biotinylated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human albumin. This antibody may cross react with albumin from other species. Goats were immunized with purified human albumin of serum or plasma origin. Antiserum was solid phase adsorbed to ensure specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human albumin. This antibody may cross react with albumin from other species. Goats were immunized with purified human albumin of serum or plasma origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human albumin. This antibody may cross react with albumin from other species. Goats were immunized with purified human albumin of serum or plasma origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human albumin. This antibody may cross react with albumin from other species. Goats were immunized with purified human albumin of serum or plasma origin. The antiserum was fractionated and passed over DEAE to yield an IgG fraction.
By immunoelectrophoresis, the antiserum reacts specifically with human albumin. No antibody was detected against other serum proteins.
Additional Product Information
Albumin is a serum protein responsible for pH maintenance and the transport of a variety of compounds, such as fatty acids, amino acids, and hormones. It is also capable of binding to water molecules and modulates oncotic pressure.
Alternate Names
HSA; PRO0883; PRO0903; PRO1341; serum albumin
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP), double diffusion Ouchterlony (DD) or immunofixation electrophoresis (IFE)).
Optimal working dilutions should be determined experimentally by the investigator.