Goat anti-Human Alpha-1-Antitrypsin Antibody Biotinylated
Product Details
Specifications
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Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with alpha-1-antitrypsin. No antibody was detected against other serum proteins. This antibody may cross react with alpha-1-antitrypsin from other species. Goats were immunized with purified human alpha-1-antitrypsin of serum or plasma origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Alpha-1-Antitrypsin. No antibody was detected other immunoglobulin serum proteins. This antibody may cross react with alpha-1-antitrypsin from other species. Goats were immunized with purified human alpha-1-antitrypsin of serum or plasma origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with alpha-1-antitrypsin. No antibody was detected against other serum proteins. This antibody may cross react with alpha-1-antitrypsin from other species. Goats were immunized with purified human alpha-1-antitrypsin of serum or plasma origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with Alpha-1-Antitrypsin. No antibody was detected other serum proteins. This antibody may cross react with alpha-1-antitrypsin light chain from other species. Goats were immunized with purified human alpha-1-antitrypsin of serum or plasma origin. Antiserum was solid phase adsorbed to ensure specificity. The antiserum was fractionated and passed over DEAE to yield an IgG fraction.
By immunoelectrophoresis this antibody reacts specifically with alpha-1-antitrypsin. No antibody was detected against other serum proteins. This antibody may cross react with alpha-1-antitrypsin from other species.
Additional Product Information
Alpha-1-Antitrypsin (A1AT) is a liver-derived protein found in the serum that functions to inhibit enzymes released by inflammatory cells. Deficiencies can lead to tissue damage in the lung or the liver in the event that secretion is inhibited via mutation.
Alternate Names
A1A; A1AT; AAT; alpha-1 antitrypsin; alpha-1 protease inhibitor; alpha-1-antiproteinase; alpha-1-antitrypsin; alpha-1-antitrypsin null; alpha-1-antitrypsin short transcript variant 1C4; alpha-1-antitrypsin short transcript variant 1C5; alpha1AT; epididymis secretory sperm binding protein; nNIF; PI; PI1; PRO2275; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1; serpin A1; serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1; serpin peptidase inhibitor clade A member 1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP), double diffusion Ouchterlony (DD) or immunofixation electrophoresis (IFE)).
Optimal working dilutions should be determined experimentally by the investigator.