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Goat anti-MED1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
MED1
Reactivity:
Human, Mouse
Host:
Goat
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1531 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CRSP1/TRAP220 immobilized on solid support.

The epitope recognized by A303-876A-T maps to a region between residue 1531 and 1581 of human Mediator complex subunit 1 using the numbering given in entry NP_004765.2 (GeneID 5469).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to MED1 immobilized on solid support.

The epitope recognized by A303-876A maps to a region between residue 1531 and 1581 of human Mediator complex subunit 1 using the numbering given in entry NP_004765.2 (GeneID 5469).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15648].

5469
MED1
mediator complex subunit 1
Q15648
Mediator of RNA polymerase II transcription subunit 1

Alternate Names

activator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; Mediator complex subunit 1; mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; peroxisome proliferator-activated receptor-binding protein; PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyroid receptor-interacting protein 2; TRAP220; TR-interacting protein 2; TRIP2; TRIP-2; vitamin D receptor-interacting protein 230 kD

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).