Bethyl Laboratories
Goat anti-MED1 Antibody Affinity Purified
Primary
Catalog #A303-876A
TargetMED1
HostGoat
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15648].
Specifications
Clonality
Polyclonal
Host
Goat
Immunogen
Between 1531 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMED1
Antigen Species
Human
Gene ID5469
Gene Symbol
MED1
Gene Name
mediator complex subunit 1
Uniprot IDQ15648
Protein Name
Mediator of RNA polymerase II transcription subunit 1
Alternate Names
activator-recruited cofactor 205 kDa component • ARC205 • CRSP1 • CRSP200 • DRIP205 • DRIP230 • Mediator complex subunit 1 • mediator of RNA polymerase II transcription subunit 1 • p53 regulatory protein RB18A • PBP • peroxisome proliferator-activated receptor-binding protein • PPAR-binding protein • PPARBP • PPARGBP • RB18A • Thyroid receptor-interacting protein 2 • TRAP220 • TR-interacting protein 2 • TRIP2 • TRIP-2 • vitamin D receptor-interacting protein 230 kD
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MED1 immobilized on solid support.
The epitope recognized by A303-876A maps to a region between residue 1531 and 1581 of human Mediator complex subunit 1 using the numbering given in entry NP_004765.2 (GeneID 5469).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:500 - 1:2500
Documents
Product DatasheetPDF · A303-876A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-876A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-876AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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