Bethyl Laboratories
Goat anti-Pumilio 1 Antibody Affinity Purified
Primary
Catalog #A300-201A
TargetPumilio 1
HostGoat
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Pumilio 1 is an evolutionarily conserved RNA binding protein that functions as a translational regulator of genes involved in embryogenesis and germline cell development. Pumilio 1, which is expressed in many tissue types, is homologous to the germline specific pumilio 2.
Specifications
Clonality
Polyclonal
Host
Goat
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPumilio 1
Antigen Species
Human
Gene ID9698
Gene Symbol
PUM1
Gene Name
pumilio RNA binding family member 1
Uniprot IDQ14671
Protein Name
Pumilio homolog 1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Pumilio immobilized on solid support.
The epitope recognized by A300-201A maps to a region between residues 225 and 275 of human Pumilio 1 using the numbering given in entry NP_055491.1 (GeneID 9698).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-201A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-201A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-201AOpen
Citations / Publications
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