Bethyl Laboratories
Goat anti-RFC1 Antibody Affinity Purified
Primary
Catalog #A300-141A
TargetRFC1
HostGoat
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC1 is the 140 kDa subunit of RFC that has a DNA-binding domain with a BRCT motif and is suggested to play a more critical role than the other subunits.
Specifications
Clonality
Polyclonal
Host
Goat
Immunogen
Between 1098 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRFC1
Antigen Species
Human
Gene ID5981
Gene Symbol
RFC1
Gene Name
replication factor C subunit 1
Uniprot IDP35251
Protein Name
Replication factor C subunit 1
Alternate Names
A1 • A1 140 kDa subunit • activator 1 140 kDa subunit • activator 1 large subunit • activator 1 subunit 1 • DNA-binding protein PO-GA • MHC binding factor, beta • MHCBFB • PO-GA • RECC1 • replication factor C (activator 1) 1, 145kDa • replication factor C 140 kDa subunit • replication factor C large subunit • replication factor C subunit 1 • replication factor C1 • RFC • RF-C 140 kDa subunit • RFC140
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RFC1 immobilized on solid support.
The epitope recognized by A300-141A maps to a region between residue 1100 and the C-terminus (residue 1148) of human Replication Factor C subunit 1 using the numbering given in entry NP_002904.3 (GeneID 5981).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-141A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-141A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-141AOpen
Citations / Publications
Services
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