Bethyl Laboratories
Goat anti-RFC2 Antibody Affinity Purified
Primary
Catalog #A300-142A
TargetRFC2
HostGoat
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC2 is the 40 kDa subunit of RFC that is part of the core complex formed with RFC4 and RFC5.
Specifications
Clonality
Polyclonal
Host
Goat
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRFC2
Antigen Species
Human
Gene ID5982
Gene Symbol
RFC2
Gene Name
replication factor C subunit 2
Uniprot IDP35250
Protein Name
Replication factor C subunit 2
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RFC2 immobilized on solid support.
The epitope recognized by A300-142A maps to a region between residues 1 and 50 of human Replication Factor C subunit 2 using the numbering given in entry NP_852136.1 (GeneID 5982).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).
Western Blot (WB)1:500 - 1:5000
Documents
Product DatasheetPDF · A300-142A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-142A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-142AOpen
Citations / Publications
Services
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