Bethyl Laboratories
Mouse anti-BRD2 Monoclonal Antibody Affinity Purified [12C2E4]
Monoclonal Antibodies
Catalog #A500-014A
TargetBRD2
HostMouse
Clone12C2E4
ReactivityHuman
ApplicationIP, WB
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Product Description
BRD2 (bromodomain-containing 2) is a nuclear mitogen-activated kinase that plays a role in transcription of cell-cycle-regulated genes by functioning as an adapter protein that recruits E2F and chromatin remodeling factors to E2F response elements.
Specifications
Clonality
Monoclonal
Clone
12C2E4
Host
Mouse
Immunogen
Between 675 and 725
Isotype
IgG1, kappa
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
40 µg/ml
Target Identity
TargetBRD2
Antigen Species
Human
Gene ID6046
Gene Symbol
BRD2
Gene Name
bromodomain containing 2
Uniprot IDP25440
Protein Name
Bromodomain-containing protein 2
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific for BRD2 immobilized on solid support.
The epitope recognized by A500-014A maps to a region between residue 675 and 725 of human bromodomain containing 2 using the numbering given in entry NP_005095.1 (GeneID 6046).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A500-014A · Lot 3 (current lot)Open
Product DatasheetPDF · A500-014A · Lot 3 (current lot)Open
Product DatasheetPDF · A500-014A · Lot 3 (current lot)Open
Safety Data SheetPDF · A500-014AOpen
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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