Bethyl Laboratories
Rabbit anti-AATF/Che-1 Antibody Affinity Purified
Primary
Catalog #A301-031A
TargetAATF/Che-1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
AATF/Che-1 was identified as a protein that interacts with RNA polymerase II and is involved in the regulation of gene transcription and cell proliferation. AATF/Che-1 has been demonstrated to promote cell proliferation by acting as a competitor of histone deacetylase 1 (HDAC1) and interfering with HDAC1’s interaction with the growth inhibiting protein pRB. AATF has also been shown to function as a nuclear receptor coactivator and a repressor of apoptosis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 125 and 175
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetAATF/Che-1
Antigen Species
Human
Gene ID26574
Gene Symbol
AATF
Gene Name
apoptosis antagonizing transcription factor
Uniprot IDQ9NY61
Protein Name
Protein AATF
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AATF/Che-1 immobilized on solid support.
The epitope recognized by A301-031A maps to a region between residue 125 and 175 of human apoptosis antagonizing transcription factor (Che-1) using the numbering given in entry NP_036270.1 (GeneID 26574).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-031A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-031A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-031AOpen
Citations / Publications
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