Bethyl Laboratories

Rabbit anti-ABCF3 Antibody Affinity Purified

Primary
Catalog #A302-495A
TargetABCF3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
Detection of human ABCF3 by western blot and immunoprecipitation.
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Product Description

ATP-binding cassette sub-family F member 3 (ABCF3) contains two ABC transporter domains. Some members of the ABC transporter superfamily play a role in transport of physiologic substrates and are responsible for chemotherapy drug exclusion. ABCF3 lacks transmembrane domains and is probably not involved in transport [Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NUQ8].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetABCF3
Antigen Species
Human
Gene ID55324
Gene Symbol
ABCF3
Gene Name
ATP binding cassette subfamily F member 3
Uniprot IDQ9NUQ8
Protein Name
ATP-binding cassette sub-family F member 3
Product Information
Applications
WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to ABCF3 immobilized on solid support.

The epitope recognized by A302-495A maps to a region between residue 1 and 50 of human ATP-binding cassette, sub-family F (GCN20), member 3 using the numbering given in entry NP_060828.2 (GeneID 55324).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:2000 - 1:10,000

Documents

Product DatasheetPDF · A302-495A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-495A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-495AOpen
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