Bethyl Laboratories

Rabbit anti-ABRO Antibody Affinity Purified

Primary
Catalog #A301-256A
TargetABRO
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Detection of human ABRO by western blot of immunoprecipitates.
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Product Description

ABRO is a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. The function of ABRO has not been characterized.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 365 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetABRO
Antigen Species
Human
Gene ID23172
Gene Symbol
ABRAXAS2
Gene Name
family with sequence similarity 175 member B
Uniprot IDQ15018
Protein Name
BRISC complex subunit Abro1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to ABRO immobilized on solid support.

The epitope recognized by A301-256 maps to a region between residue 390 and 415 of human abraxas brother 1 using the numbering given in entry NP_115558.3 (GeneID 23172).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A301-256A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-256A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-256AOpen

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