Bethyl Laboratories
Rabbit anti-AF4 Antibody Affinity Purified
Primary
Catalog #A302-345A
TargetAF4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
AF4 is a member of the AF4/LAF4/FMR2 (ALF) family of transcription cofactors. It has been identified as part of a translocation [t(4:11)(q21;q23)] found in patients with acute leukemia involving MLL/HRX. AF4 functions as a cofactor of transcriptional elongation and chromatin remodeling.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 175 and 225
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAF4
Antigen Species
Human
Gene ID4299
Gene Symbol
AFF1
Gene Name
AF4/FMR2 family member 1
Uniprot IDP51825
Protein Name
AF4/FMR2 family member 1
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AF4 immobilized on solid support.
The epitope recognized by A302-345A maps to a region between residue 175 and 225 of human proto-oncogene AF4 using the numbering given in entry NP_005926.1 (GeneID 4299).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-345A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-345A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-345AOpen
Citations / Publications
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